best foods

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Which is the best food? Some say rice is the best food. About half of the population of the world are dependent on rice grain for energy. Principal constituent of rice is carbohydrate which is the principal source of energy of the most of the Asian people. It contains about 5% protein.


The rice satisfies our hunger very efficiently as well as it is bulky to our intestine too which eliminates air from our alimentary canal. Its intake also makes us feel we are satisfied since it fills every corner of our stomach very efficiently. So people say rice is the best food in the world. Some say brown rice is the best food. Some say white rice is the best food.

For me, rice with pulse and green vegetable is the best food meal. It is because I have eaten the meal since I was young.  I am habituated in the meal. So the rice is the best food. After long duration traveling, I extremely desire to take rice with pulse and cooked vegetables or leafy vegetables. When I complete taking the meal, I feel extreme satisfaction. So, I say rice is the best food.

Japanese people too like rice. So I say rice is the best food for Japanese people. Majority Japanese people like rice very much. They eat rice in the time of breakfast, lunch and dinner. They say breakfast "Asa Gohan". In Japanese language, 'Asa' is meant for 'morning' and Gohan is meant for cooked rice grains which is prepared cooking rice grains with water in rice cooker. Sometimes in party, they take Japanese drink with rice ball after the rice grains are cooked. Generally, rice is eaten at least three times in a day in Japan.  So I say rice is the best food in Japan.

They make Sweets which they call "Okhasi" in their language as the Japanese sweets. Japanese sweets are generally made from the cooked rice grains. Japanese traditional alcoholic drinks are also made from the rice grains. They call the alcoholic drink 'Sake' or 'Osake'. Osake is made from central region of the rice grains.  So rice is a versatile and best food item in Japan.

Majority of Japanese farmers generally prefer growing rice in their farms although growing other crops  such as strawberry are more profitable than growing rice. For example; growing strawberry in their field gives more returns than  the returns from the rice crop. It is traditional way of livelihood which majority of Japanese farmers do not want to stop. Although Japan is the most industrial country in the world, most of the farmers like to grow traditional crop: rice in their farms in Japan. From these views, I like to say that rice is the best food in Japan.

Japanese scientists have been vigorously  working in many different fields of the rice crop science than any other country crop scientists in the world. They have allocated more funds in the rice research than any other crop. So, I say rice is the best food crop in Japan.

In Indonesia, rice crop is the principle crop of farming system of the country. Although farmers know shrimp cultivation can give them three times more return than rice crop, the majority of Indonesian farmers could not stop growing rice to raise shrimp in their farmland.If they leave cultivating rice in their farmland, then Indonesia has to import rice grain in high price to feed its people since rice is the principal staple food of Indonesia. So, I can write here rice is the best food in Indonesia.

Let us discuss about some other foods too. Some say Chapati made from wheat flour is the best food. Some say bread made from wheat flour is the best food in the world. They say that the Chapati made from wheat flour is full of fiber, rich in calories and body building molecules "protein." So they say Chapati is one of the best foods.

I furthermore admire the beauty of the Chapati made from bread wheat. Its intake in the meal slowly releases calories to  our blood, brain and body. Sudden glucose swing from the meal is not good, but slow and steady glucose level in the blood from the intake of the meal of the Chapati forces me to say that Chapati made from wheat flour is one of the best foods in India.


The wheat flour has some more variety of proteins than rice and it constitutes about 10% protein quantity in contrast to the 5% protein of the rice grains. Thus protein content of wheat flour is two times more than rice protein amount. Some say fried rice is the best food in the world. The wheat flour is cheaper but of higher quality than rice. Generally, people in India, Pakistan, Bangladesh eat the Chapati. So, they say Chapati is the best food in South Asia.


Some say bread made from whole wheat flour is the best food. There are a variety of breads. Sliced bread is  one of the most popular food items among most of European and North American people. Some like biscuits. Some say pancake made from wheat flour is the best food. Some say pizza which is made from the wheat flour is the best food.

Some say milk is the best food in the world with respect to the availability of minerals, calories, protein and vitamins. Some people think it is complete food. But, I don't think it is complete food. Taking milk alone we cannot be comfortable since it is rich in saturated fats. The saturated fats present in the milk are not so good, but the plant origin unsaturated oils is much healthier.

And the carbohydrate in the form of lactose in the milk is not enough to fulfill the calorie requirement of the adult people. Brain requires unsaturated fats for its development as well as functioning. So, I say the milk is not the complete diet and it is not best food in the world. Now, health conscious people in Finland started talking with a slogan, "Leave Diary, Start Berry."

Some say pulse soup is the best food. Mungbean is rich in carbohydrates and protein. But; still, mungbean is popular in protein content. It contains protein which is about four times more than the protein amount of rice and two times more than that of protein content of wheat flour. In other words, the mungbean contains about 20% protein. There is a variety of the mungbean. One of its kinds is green mungbean.

The soup made from half cut husk rich mungbean is recommended to healthy to sick people, from babies to old age people. Typhoid patients can drink the green mungbean soup very safely. The intake of the mungbean soup does not cause flatulence. Soup made from the green mungbean is the best food with respect to absence of saturated fats. That is why mungbean is said as one of the best foods.


Some say maize chapatti is the best food. When I was very young, it is about 4 to 7 years; my grandmother used to make thick Chapati from corn flour on a iron pan with simmering flame of firewood. Then she used to feed me. I remember that she wanted to include diversified food items to make us satisfied. The fresh meal kinds made from corn flour is rich in carbohydrates, protein and fats. The corn is packed with enough energy and brain food nutrients. I think foods made from the corn are some of the best foods.


Some say fish is the best food. Some say chicken is the best food. Some say chevon is the best food in the world. Some say meat of ostrich is the best food in the world. Some say beef is the best food in the world.

Some say Momo “love ball” is the best food in the world. Some say green vegetables such as asparagus, broccoli, and tomato are the best food. Some say onion and garlic mixed vegetable dish are the best foods in the world.

Some say fish is the another best food item.  Some people consider filter coffee is the best food between the two drinks: green tea and coffee. Some say green tea is the best drink. Some say coffee is the best food. Green tea is recommended to the hypertensive people, but coffee is recommended to hypotensive people. I too think in this way. Some say Matcha is the best food. Please remember Matcha is a special preparation of the green tea which I also think as the best food if we compare it with coffee, coca, coffee, ordinary tea and green tea.

Fruits like mango, black grapes, and apple are also some of the best foods. Berries such as acai berry are some of the best foods. Some people are allergic to some fruits. Asthmatic patients should avoid banana. But, banana is one of the best foods for majority of people in tropical and subtropical countries.

Most of the children in the world like chocolate. When they are asked, "What is the best food?" They mostly answer,  "Chocolate is the best food. " Mostly chocolate is made mixing cocoa, sugar, milk ingredients. Food rich in sugar is not so good for the teeth health. I also advice to the children of the world, "Please don't take chocolate."  Accordingly, I too state chocolate is not the best food.

By the way, cocoa is brain food. So, it is considered one of the best food items. But cocoa formulated in the form of chocolate is not so good. If cocoa is formulated with dairy product such as butter or milk and sugar is not best absorbed through the human intestine of ours. So, it is not considered the best food.

Dairy fat is saturated animal fat, so its continuous intake is not so good to the body of all age people. Sugar like sweetening substance is not so beneficial to teeth health, diabetic and hypertensive people. The cocoa with soybean milk and stevia sweetening substance is best recommended one of the best foods in the world.

Some people like alcoholic drinks. So they say alcoholic drinks are some of the best foods. Some like non-alcoholic drinks.  So they say non alcoholic drink such as lemonade, coconut water are some of the best foods. Some say brandy is the best food. Some say whiskey is the best food. Some say wine is one of the best foods.  Some say orange fruit juice is the best food. Some say grape juice is the best food. Best food varies from person to person and state to state.


Some say Lassi drink is one of the best foods. By the way, Lassi is made from yoghurt mixing with small cardamon powder, rose water, ice, and sugar. It is generally drunken in hot summer days in India. Majority people feel special satisfaction drinking Lassi in summer days. So, I too say Lassi is one of the best food substance in hot days in tropical climatic countries.

Every country and continent people have their own staple food. People in high altitude Himalaya do have potato as the staple food. Low mountain people in Nepal have maize as the best food.

Thank you very much for the visit to the page of the "Best food group: Best food forum: Best food discussion group forum."

fish recipes: fish dish

This is the page of fish recipe or fish recipes or fish dish. You are welcome in the page of the fish recipe or fish recipes or fish dish. Fish is the best food to all people in the world. Furthermore, fish is available in tropics to extreme temperate region in the world. Fish protein is very digestible to the human intestine of all age group. Fish fat is ranked as the first grade fat in the food list of ours.  So  our fish recipes must  be modified to protect the nutritive value of the fish.

There are a variety of fish. The fish recipe must be made based on the  fish kind. I classify fish by three classification criteria. One classification is based on the water type. One kind of the fish is fresh water fish. Another kind of fish is sea water fish.  The sea water fish can also be called as marine fish. So our fish recipes must be refined to protect the health value of the  fish.

There is another kind of fish classification based on the kind of backbone of the fish. One kind is cat fish and another is non-cat fish. So we must consider the the fish recipe to match the bone type of fish.  There is still another useful classification of fish: scale fish and non-scale fish.This classification give us idea about how to cut the fish to make into fish pieces.

Fish recipe 1

Ingredients for the fish recipe 1: Fish 1 to 1.5 kg, Tomato 250 grams, onion 200 grams, garlic 50 grams, turmeric 2+2 grams, salt 3+ 3 grams, coriender 5 grams, cumin 5 grams, mustard grains 25-50 grams, water about 300 ml, olive oil 50-100 ml.

Step 1: Spice paste 1: First take some rape grains or mustard grains. Grind grains so as to make it paste.

Step 2: Spice paste 2: Similarly,  Cut onion after  removing the outer skin of the onion, then clean the onion bulbs, chop the onion into pieces. Wash tomatoes and cut into the pieces. Separate some garlic fingers. Using grinding machine or blending machine, make paste of onion pieces, garlic pieces and tomato pieces.

Step 3: Make misture 3. For it, mix some coriender grain powder and cumin grain powder or make paste grinding the cumin and coriender grains.

Step 4: Spice paste 4: Make mixture of salt and turmeric. Don't put too much turmeric into the mixture. It is the mixture of salt and turmeric.

Step 5: Fish cleaning and cutting: In the fresh water scale fish recipe, wash the fish, remove the scales of the fish. First  cut the fish to make into two pieces just below the head region. Then remove the alimentary canal. If the fish contains eggs, take out and place the eggs separately.  Wash the fish to wash the abdominal region cavity of the fish. Then, cut the fish into pieces and put them into a utensil or a vessel.

Step 6: Thereafter, add mixture of salt and some turmeric powder onto the fish pieces and mix the fish pieces with the  mixture of turmeric and salt. Don't add salt too much. If you are the patients of high blood pressure, you better not add salt. Instead of salt, you add fresh lemon juice into the fish dish after it is cooked or before you eat the fish dish.

If it is the fish recipe of marine fish, we don't need to add salt to the fish pieces. But, we have to add salt to the paste 1 and paste 2. If we are making fish dish adopting the fish recipe 1, then we have to add salt into fish pieces at step 6 and step 8 for the fish recipe 1.

Step 7: By adopting any one method of cooking pot, we can use the fish recipe to make the fish dish. We can use a steam cooker or a cooking pan. Now we are using a cooking pan. Put the pan onto the stove flame.  Then, add some plant oil which you have used to add in your country as a cooking oil. Olive oil is the best cooking oil. But; peanut oil, or flax seed oil or other unsaturated plant origin oil is optional for the fish recipe.

Step 8: For the fish recipe 1, we first add paste 1 into the cooking pan. Then we cook the paste 1 in simmering flame. When the paste change slightly red, then we add paste 2. Then again, we cook the both the paste together. Then again we add paste / mixture 3. When  the final paste looses about half of the moisture present in the paste 2.

Step 9: Then add the fish pieces into the cooking pan. Then, mix the fish pieces with the paste with the help of a spoon.

Step 10: Add some water. And cover the cooking pan with its lid. We give high flame.  We wait until it boils.  Then, we lower the flame at medium level with its lid cover. The stove flame should not very high, nor very low. We wait about 10 minutes for enough cooking. Thereafter, we can taste whether fish pieces are cooked or not. I think the fish dish with the fish recipe is ready.

We can serve. We can take rice with the thick soup of the fish dish. The food with fish dish can be used as a meal. It can be a breakfast, or lunch or dinner.

Please write how delicious the fish dish is using the fish recipe 1.

Alternative of the cooking pan: Steam cooker

If we use the recipe to cook in a steam cooker, we can do the same thing as we did in the cooking pan. In the steam cooker, we make airtight with the steam cooker lid while after mixing the fish pieces with the cooked paste and adding some water to dip almost all fish pieces. We should give high flame until the cooker give two whistles. Just after 3 minutes, we can open the cooker lid cooling the steam cooker in tap water. the fish dish is ready. We can serve similarly as we serve the fish dish cooked in the cooking pan.

Please write how delicious the fish dish is using the fish recipe 1.

Fish recipe 2

11) The fish recipe 2 very simple. Make the fish into pieces as mentioned in the steps 5 and mix the mixture of salt and turmeric as mentioned in step 6. Make the fish pieces somewhat larger than the step 5 of the fish recipe 1.

12) Cook the fish pieces in an oven. If you have microwave oven, cooking fish in microwave oven is better than the ordinary oven.  Fix the microwave oven in microwave mode. We can cook the fish pieces into the microwave oven in both the modes: microwave and grill. Fix the temperature and duration as mentioned in the catalogue of the microwave oven.

The fish dish is ready with the fish recipe 2.

Fish recipe 3: Fish recipe varies with the kind of fish too. Fish recipe also varies from country to country. One most important thing  is that fish recipe should not be fried in deep oil. The brain fuel of human being is lost if the deep frying of the fish is done.

Thanks for the visit of the fish recipe or fish recipes or fish dish.

chicken recipes

This is the page of the chicken recipe or chicken recipes. I welcome you in the page of the chicken recipe or chicken recipes. The chicken recipes are mostly common in India, Nepal, Pakistan and Bangladesh. How did you find the chicken dish with the chicken recipes, please write me.

Chicken recipe 1: The chicken recipe 1 is also called as Chicken chilly recipe.

Step 1: Ingredients required for the chicken recipe 1: -

Step 2: Cut pieces of chicken 1 kg; Onion 300 grams, Garlic : one onion bulb, Capsicum: 3 - 4 capsicum fruit, Spice powder of cumin and coriender 5-10 grams.

Step 3: For the chicken recipe 1, chop onion into pieces after washing with clean water after removing outer skin of the onions. Crush some garlic fingers too. Cut a capsicum into pieces too. Capsicum pieces and onion pieces should be of about square shape.

Step 4: For the chicken recipe 1, cut the chicken into pieces. The size of the chicken pieces can range from 50 grams to 100 grams. If it is possible, remove all the skin of the chicken pieces.  You can also buy chicken without skin, bone and unnecessary fats.

Step 5: For the chicken recipe 1, put the cooking pan onto the stove flame. Put edible plant origin cooking oil into the pan.  After the heating of the cooking oil, put the cut chicken pieces  into the cooking pan. We better add some common salt, turmeric. Put the lid of the cooking pan onto the cooking pan to cover it. Cook the chicken pieces until it fully cook. In other words, I can say, cook the chicken pieces until they become slightly red. In other words, cook the chicken pieces to eliminate all the visible water in the cooking pan. Add the spice powder.

Step 6: When the cut chicken pieces become cooked, add the cut pieces of the onion, cut pieces of the capsicum and  crushed garlic into the cooking pan. Cook them. Instead of the turmeric, we can add coloring substance too. But, the coloring substance should be plant origin such as Amaranthus red coloring substance.

Step 7: Cooking is continued just to eliminate the hardness of the onion pieces. Or in other words, cooking is done just kill the pungency of the onion pieces. We can use saffron too in place of coloring substance. If we are putting saffron, we better put it after putting the chicken pieces pieces become fully cooked. At the end, add some tomato ketchup and vinegar. After a while the chicken dish of chicken chilly becomes ready.

Chicken Recipe 2:

For the chicken recipe 2;

Plant biotechnology and Molecular Markers in Nepal

Introduction

Molecular markers generally used are restriction fragment; southern hybridization; restriction fragment length polymorphism; polymerase chain reaction based markers such as DNA amplification fingerprinting, random amplified fragment length polymorphism, simple sequence repeats, cleaved amplified fragment length polymorphism. The isozyme based protein gel electrophoresis technique which has been exploited in the breeding and genetic study of our economically important crop species is one of the most popular biochemical markers.

History of molecular markers in Nepal

Plant biotechnology also includes regeneration of plants from cells, or tissue or organ, or embryo or other parts through the test tube culture in laboratories. The cell may have foreign gene transferred in the laboratory which is known as transgenic cells. The plants regenerated from the transgenic cells are known as transgenic plants. Plant biotechnology also includes technology of production of new plants or plants products using technology. The plant biotechnology also includes use of extraordinary use of DNA for a variety of purposes including plant breeding.

First time, the plant tissue cultured was started in Nepal in 1976 at the Nepal government’s National Herbarium and Plant Research Laboratory in Godawari. This laboratory basically has been running clonal propagation of hard wood trees, horticulture crops, medicinal plants, ornamental plants and over 30 kinds of orchids. The scientists in the laboratories have been working in some anther culture as well as some chromosome works in their high interests.

One private laboratory called as RLABB (Research Laboratory for Agriculture Biotechnology and Biochemistry) funded by Rockfeller Foundation of the USA was established in 1986 in Kathmandu, Nepal. The Scientist V. N. Agrawal pioneered a work in regeneration of cold tolerant rice from Jumli Marshy through anther culture technology. He also worked in agriculture crop species and forest tree species using some molecular markers. This lab had also worked in the area of sex-determination of dioecious plant Lapsi and micropropagration of many plants like pinus, Autocarpous, Rice, orchids, etc. Some other private enterprises (Botanical Enterprise, Nepal Biotech Nursery, Himalayan Floratech, Microplant Nepal) have also working in rapid propagation of orchids using tissue culture techniques.

Potato Development Centre at Khumaltar has been working on tissue culture of potato to regenerate and multiply virus free clone of potato. Basic research on tissue culture was also initiated at Central Department of Botany, Kritipur mainly of medicinal activities. The graduate students have also working in some agricultural, medicinal, ornamental and forestry crops and trees under the supervision of senior level professors in Department of Botany of Kirtipur Tribhuvan University, Kathmandu, Nepal.

A new biotechnology laboratory Green Research Technology was established which has been working in the regeneration of virus free plants of Citrus, Cardamom and potato plants using meristem culture in Kathmandu, Nepal. Laboratory for Agriculture Biotechnology and Biochemistry, Sanepa, Potato Development Centre at NARC, and Central Laboratory of Rampur Campus of Institute of Agriculture and Animal Science, Rampur (IAAS, Rampur, Chitwan, Nepal) of Tribhuvan University have  some basic facilities to conduct scientific molecular marker based works.

Micro plant Nepal, Nepal Biotech Nursery, Himalayan Floratech, Nepal Biotech Nursery, Himalayan Floratech, Botanical Enterprise, Potato Development Center, Plant Research Division, Green Research and Technologies, and Central Department of Botany, Kirtipur are existing and working in in vitro plant culture for different species of crop species of economic importance and poverty alleviation.

Nepal Biotechnological Association (NBA) and Nepal Molecular Biology Society (NEMBIS) are organizations working in the promotional developmental and commercial applications of research findings in the field of plant biotechnology.

Molecular markers in Plant Breeding in Nepal

No routine and regular works are in the process of molecular map fingerprinting in any government and private owned laboratories in Nepal. Ministries of Education, Science, Technology, and Agriculture are not interested to allocate enough and regular funds to the government laboratories for exploitation of molecular markers based plant breeding. Some irregular works are going on sporadically in some crops.

Utilization of Molecular Markers in Plant Breeding

Utilization of molecular markers in plant breeding takes place in two step works. First, construction of high density of molecular markers of our crop species and then; utilization of the molecular markers in marker based selection and breeding of our crops. Nepal cannot allocate for construction of high density chromosome map of the genome of a few very important economically important crops such as rice, wheat, maize, potato. Plant biotechnology laboratories in Nepal can exploit the information of the high density genome mapping of the crops worked in developed countries and published in the international plant genome related publishing journals.

Molecular Markers Technology in Nepal

Molecular markers technology consists of creation of a variety of restriction enzymes; construction of cloning vectors such as plasmid, phage, yeast artificial chromosomes (YAC); cloning technology; DNA x DNA hybridization; DNA x RNA hybridization; DNA amplification through polymerase chain reaction (PCR); DNA sequencing; primer construction technology; labeling and detection technology. High density map construction of our crops is very sophisticated and expensive molecular marker development technology which we cannot do in the neglected field in the laboratories of Nepal. But, developed resource rich countries have been working in the construction of high density chromosome mapping. We can get sequence information and buy primers to amplify the target DNA region to develop marker based selection breeding for PCR based marker assisted selection and breeding works of our crops. High density mapping of wheat, rice, maize, sorghum have been constructed in the resource rich countries like the USA, Japan, UK, France in coordinated projects.

Isozyme and DNA Fractionation as molecular markers in Nepal

Plant protein analysis is performed through the protein gel electrophoresis technique. DNA Amplification and DNA fractionation has been performed in few laboratories in Nepal. But the such works are not regular in the laboratories since the government has not been regular to allocate funds for such works.

DNA hybridization fingerprinting in Nepal 

Scientists are interested to do works using DNA hybridization fingerprinting technique; but they are not getting enough funds and regular electric supply to do such works in Nepal. Still some sporadic works are going on in some private and government laboratories.

DNA amplification fingerprinting in Nepal

DNA amplification fingerprinting have been done through PCR (polymerase chain reaction) in some laboratories in some crop species such as rice, cardamom; but the works are not regular in the few laboratories in Nepal. Biotechnology Laboratory of Nepal Agriculture Research Council, Khumaltar-Lalitpur, Nepal has become a leading laboratory in Nepal in recent days (2012).

Marker assisted selection based breeding

No any crop has been developed through the marker assisted selection breeding techniques in Nepal yet.

Use of molecular markers in Nepal

Professor Dr. R.C. Sharma worked to develop blast resistant rice for Nepalese farmers. For it, he got some funds from Rockfeller Foundation of the USA to work in collaboration with IRRI scientists (International Rice Research Institute, Los Banos, Philippines). Dr RC Sharma worked on blast resistance of popular Nepalese rice Laxmi and introduce pyramid blast resistance genes into popular Nepalese rice: Masuli. Post-doctorate plant pathologist, Dr. Tika B. Adhikari also worked in bacterial blight pathogen of rice using RFLP, PCR based DNA fingerprinting techniques  in Nepal and Kansas University of the USA during 1990s in the funding of Rockfeller Foundation. He did some part of it at IAAS, Rampur-Chitwan and some part his research at Kansas University in the USA. He used both radio-active isotopes and multi-color color staining detection techniques.

Breeding of Rice 

Few scientists are working in characterization of submergence genes in wild rice of Nepal in Biotechnology Lab of Nepal Agricultural Research Council (Personal communication with B. Tamang, 2012)

Breeding of Maize 

No any molecular markers have been used for the improvement of the maize crop. Conventional breeding and evaluation of the local and exotic germ plasm have been going on the crop of the maize in Nepal.

Breeding of Wheat 

Few scientists have been working in exploration of leaf rust, stem rust and yellow rust resistance genes in Nepalese bread wheat at Biotechnology Lab of Nepal Agricultural Research Council (Personal communication with S. Acharya Sharma, 2012). Sharma worked on previously identified gene sequence based primer information of the rust resistance of the wheat.

Breeding of Lentil (Lens culinaris)

Few scientists have been working in the study of genetic and morpho-physiological diversity exploration of lentil genotypes of Nepal and ICARDA through Simple Sequence Repeat (SSR) markers and natural field based morpho-physiological phenotypes at the laboratory of plant biotechnology of Nepal Agricultural Research Council (Personal communication with U. Kushwaha, 2012).

Breeding of Arahar Cajanus cajan 

No molecular markers based works have been applied in any department in Nepal to study and exploit the science of Cajanus cajan in Nepal. Most of the works are selection activities of the crop to breed promising cultivars of arahar.

Breeding of Potato

Plant Biotechnology laboratory of Nepal Agriculture Research Council (NARC) have also worked to regenerate in vitro disease free plants and multiply planting materials of potato.

Breeding of Sugarcane

Few scientists are working in generation and rapid multiplication of virus free sugarcane through tissue culture based somaclonal variation creation technique in Nepal in Biotechnology Lab of Nepal Agricultural Research Council (Personal communication, 2012)

Breeding of Cardamom

Few scientists are working in generation and rapid multiplication of virus free cardamom in Nepal in Biotechnology Lab of Nepal Agricultural Research Council (Personal communication, 2012). They have been using ELISA test too to identify whether the regenerated plants have viral pathogen still existing.

Breeding of Strawberries

Few laboratories are regenerating disease free planting materials of exotic strawberry species in Kathmandu and Kakani, Nepal.

Breeding of Papaya:

No laboratory is working using molecular markers to study and exploit the science of the crop of papaya in Nepal. Horticulturists can apply sex determining isozyme marker to differentiate female papaya seedlings from male in early age.

Breeding of Kiwi:

Private nurseries are working to regenerate and multiply disease resistance planting materials of Kiwi fruit in Nepal. Some innovative farmers and ICMOD are also producing some planting materials of grafted type.

Breeding of dwarf orange

Dwarf orange scion has been introduced from Japan and grafted onto Trifoliate orange rootstock in Kirtipur Horticulture Farm of the government in Kathmandu, Nepal. Its multiplication in the government farm as well as private is going on.

Cooperation from the people of Japan for fruit development in Nepal

Breeding of lemon have introduced some high yielding orange, kiwi fruit, pear, germplasms into Nepal through JICA (Japan International Cooperation Association). Japanese horticultural scientists worked in Kirtipur Horticulture Farm of Government of Nepal in Kathmandu. They sometimes conducted Fruit Fair in Kathmandu with Awards to the farmers who have best fruits of apple and orange. They worked in the exchange of the fruits between Nepal and Japan.

Outside the boundary of molecular markers

Different field level farms of NARC have been working in the breeding, selection and evaluation of native and exotic genotypes of different crops by  conventional breeding. They could not adopt molecular techniques to study the science of the crops to breed them for Nepalese farmers. Some of the crops are water melons, pumpkin, cucumber, chillies-capsicum, brinjal, black mung, Masyang, cowpea, Mung bean, kidney bean, colorful beans, pea, tomato, okara, cauliflower, broccoli, mustard, rapeseed, sunflower, some more are outside the boundary of plant biotechnology and plant molecular markers. In other words, they are inside fence of conventional breeding and selection.   

Conclusion

Plant molecular markers and plant biotechnology have a variety of uses that help solve many problems of crop species to alleviate poverty of our Nepalese farmers. In Nepal, the agricultural yield is very low in comparison to developing and developed countries. Farmers are facing many problems like crop losses due to insects, disease, weeds, and droughts, low fertility of soil, other abiotic and biotic stresses. Also, climate change and growing population are most burning issues which must be handled to solve the problems. Food insecurity, and poor nutrition must be practically must be addressed for Nepal people.

This is prepared in June of 2012.